Processing of Thin Films Based on Cellulose Nanocrystals and Biodegradable Polymers by Space-Confined Solvent Vapor Annealing and Morphological Characteristics.

L-poly(lactic acid), poly(3-hydroxybutyrate), and poly-hydroxybutyrate-co-hydroxyvalerate are biodegradable polymers that can be obtained from renewable biomass sources. The aim of this study was to develop three types of environmentally friendly film biocomposites of altered microstructure by combining each of the above-mentioned polymers with cellulose nanocrystal fillers and further processing the resulting materials via space-confined solvent vapor annealing. Cellulose was previously obtained from renewable biomass and further converted to cellulose nanocrystals by hydrolysis with the lactic acid. The solutions of biodegradable polymers were spin-coated onto solid substrates before and after the addition of cellulose nanocrystals. The obtained thin film composites were further processed via space-confined solvent vapor annealing to eventually favor their crystallization and, thus, to alter the final microstructure. Indeed, atomic force microscopy studies have revealed that the presence of cellulose nanocrystals within a biodegradable polymer matrix promoted the formation of large crystalline structures exhibiting fractal-, spherulitic- or needle-like morphologies.

Modification of natural zeolites and their applications for heavy metal removal from polluted environments: Challenges, recent advances, and perspectives.

In recent decades, environmental pollution has become a significant problem for human health and environmental impact. The high accumulation of heavy metals in waters and soils from different sources was conducted by finding efficient and environmentally friendly treatment methods and materials for their removal. Natural zeolites have found wide-ranging applications in environmental remediation and protection, considering various treatment and modification methods designed to enhance the natural zeolites' adsorptive or ion-exchange capabilities for increased efficiency. This paper briefly consolidates the recent scientific literature related to the main characteristics of natural and modified zeolites, the advantages and limitations of their environmental remediation application, and summarizes the methodologies applied to natural zeolites in order to improve their properties. Their application for removing heavy metals from water systems and soils is also comprehensively discussed. This review highlights the excellent potential of natural zeolites to be used after specific treatment or modification as a sustainable and green material to solve numerous environmental pollution issues.

Assessment of mercury bioavailability in garden soils around a former nonferrous metal mining area using DGT, accumulation in vegetables, and implications for health risk.

Mercury (Hg) is a toxic, non-essential element for living organisms, frequently present in high concentrations in soils from industrial areas. The total, dissolved, and labile Hg concentrations in garden soils and their accumulation in edible vegetables (onion, garlic, lettuce, and parsley) grown on contaminated soils in localities situated a former mining area were evaluated. The labile Hg fraction was estimated by diffusive gradient in thin films (DGT). The soil-to-vegetable transfer factors, as well as the health risk by exposure to Hg, were calculated based on the labile Hg concentration in soil. The total Hg concentration in soil varied widely (0.11-3.77 mg kg-1), Hg in soil solution ranged between 2.14 and 20.2 µg L-1 and labile Hg between 1.13 and 18.6 µg L-1. About 36-96% (84% on average) of the Hg concentration in soil solution was found in labile form. Multivariate analysis revealed significant correlations between the labile Hg concentration in soil and Hg accumulated in vegetables. The hazard indices showed that, although the study area is affected by legacy pollution, exposure to soil and consumption of locally grown vegetables do not pose health risks.

Activated natural zeolites for beer filtration: A pilot scale approach.

A clinoptilolite-rich natural zeolite was tested as a substitute for kieselguhr as a filtering material to eliminate ingredients that cause beer haze formation. Two-grain sizes of micronized natural zeolite were thermally activated to 400 °C, to enhance its adsorption performance and remove the impurities adsorbed in the microporous system of zeolites, followed by their physicochemical characterization. The activated zeolites mixed with four commercial filter aids in different ratios were used for beer filtration at the pilot scale. Most of the physicochemical and sensory characteristics of beers filtered with commercial filter aids and with zeolites were similar. Using zeolite in filtering mixtures significantly reduces the number of microorganisms present in the filtered beer, which can eliminate the necessity of beer sterilization after filtration. The results evidenced that activated natural zeolites, which are cheap materials, are promising candidates as filter aids and can replace kieselguhr without producing any degradation of the beer filtration process.

Recovery of Precious Metals: A Promising Process Using Supercritical Carbon Dioxide and CO2-Soluble Complexing Polymers for Palladium Extraction from Supported Catalysts.

Precious metals such as palladium (Pd) have many applications, ranging from automotive catalysts to fine chemistry. Platinum group metals are, thus, in massive demand for industrial applications, even though they are relatively rare and belong to the list of critical materials for many countries. The result is an explosion of their price. The recovery of Pd from spent catalysts and, more generally, the development of a circular economy process around Pd, becomes essential for both economic and environmental reasons. To this aim, we propose a sustainable process based on the use of supercritical CO2 (i.e., a green solvent) operated in mild conditions of pressure and temperature (p = 25 MPa, T = 313 K). Note that the range of CO2 pressures commonly used for extraction is going from 15 to 100 MPa, while temperatures typically vary from 308 to 423 K. A pressure of 25 MPa and a temperature of 313 K can, therefore, be viewed as mild conditions. CO2-soluble copolymers bearing complexing groups, such as pyridine, triphenylphosphine, or acetylacetate, were added to the supercritical fluid to extract the Pd from the catalyst. Two supported catalysts were tested: a pristine aluminosilicate-supported catalyst (Cat D) and a spent alumina supported-catalyst (Cat A). An extraction conversion of up to more than 70% was achieved in the presence of the pyridine-containing copolymer. The recovery of the Pd from the polymer was possible after extraction, and the technological and economical assessment of the process was considered.

Impact of Low Lithium Concentrations on the Fatty Acids and Elemental Composition of Salvinia natans.

The photosynthetic pigments, protein, macro and microelements concentrations, and fatty acids composition of Salvinia natans, a free-floating aquatic plant, were analyzed after exposure to Hoagland nutrient solution containing 1, 3, and 5 mg/L Li. The Li content of Salvinia natans grew exponentially with the Li concentration in the Hoagland nutrient solution. The exposure to Li did not induce significant changes in Na, Mg, K, Cu, and Zn content but enhanced the Ba, Cr, Mn, Ni and Mo absorption in Salvinia natans. The most abundant fatty acids determined in oils extracted from Salvinia natans were C16:0, C18:3(n6), C18:2(n6), and C18:3(n3). The photosynthetic pigments did not change significantly after exposure to Li. In contrast, chlorophyll and protein content decreased, whilst monounsaturated and polyunsaturated fatty acids content increased after the exposure to 1 mg/L Li. The results indicated that Salvinia natans exposed to low Li concentrations may be a good source of minerals, omega 6 and omega 3.

L-Poly(lactic acid) Production by Microwave Irradiation of Lactic Acid Obtained from Lignocellulosic Wastes.

L-polylactic acid (PLA), a semi-crystalline aliphatic polyester, is one of the most manufactured biodegradable plastics worldwide. The objective of the study was to obtain L-polylactic acid (PLA) from lignocellulosic plum biomass. Initially, the biomass was processed via pressurized hot water pretreatment at a temperature of 180 °C for 30 min at 10 MPa for carbohydrate separation. Cellulase and the beta-glucosidase enzymes were then added, and the mixture was fermented with Lacticaseibacillus rhamnosus ATCC 7469. The resulting lactic acid was concentrated and purified after ammonium sulphate and n-butanol extraction. The productivity of L-lactic acid was 2.04 ± 0.18 g/L/h. Then, the PLA was synthesized in two stages. Firstly, lactic acid was subjected to azeotropic dehydration at 140 °C for 24 h in the presence of xylene, using SnCl2 (0.4 wt.%) as a catalyst, resulting in lactide (CPLA). Secondly, microwave-assisted polymerization was carried out at 140 °C for 30 min with 0.4 wt.% SnCl2. The resulting powder was purified with methanol to produce PLA with 92.1% yield. The obtained PLA was confirmed using electrospray ionization mass spectrometry, nuclear magnetic resonance, thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. Overall, the resulting PLA can successfully replace the traditional synthetic polymers used in the packaging industry.

In-situ Diffusive Gradients in thin-films passive sampling coupled with ex-situ small-sized electrothermal vaporization capacitively coupled plasma microtorch optical emission spectrometry as green and white method for the simultaneous determination of labile species of toxic elements in surface water.

This study presents for the first time the coupling between in-situ Diffusive Gradient in Thin-film (DGT) passive sampling technique and ex-situ small-sized instrumentation based on electrothermal vaporization capacitively coupled plasma microtorch optical emission spectrometry (SSETV-µCCP-OES) for the simultaneous determination of Cd, Pb, Cu, Zn and Hg in surface water. Unique features of the DGT-SSETV-µCCP-OES are low power and low Ar consumption for plasma generation (15 W, 150 mL min-1) and significant improvement of the detection limits following DGT passive sampling. The new method was validated in terms of river water analysis in comparison with graphite furnace atomic absorption spectrometry and thermal decomposition atomic absorption spectrometry. Combining the abilities of preconcentration by in-situ Chelex-DGT passive sampling with plasma microtorch equipped with a low resolution microspectrometer provided multielemental simultaneous determination with detection limits of (µg L-1) 0.01 (Cd, Zn and Hg), 0.02 (Cu) and 0.07 (Pb) in water, at least one order of magnitude better than using grab sampling without preconcentration. It was possible the quantification of labile fraction of priority hazardous metals (Cd, Pb) in river water below the instrumental limits of detection (µg L-1) of 0.12 and 0.80 obtained in SSETV-µCCP-OES without DGT sampling. The precision of the method was in the range 15.3-22.4% (combined uncertainty), while the accuracy was 95-103% and trueness of 27-33% (expanded uncertainty, k = 2). The DGT-SSETV-µCCP-OES coupling proved to be an ideal and powerful tool for surface water analysis in compliance with green and white analytical chemistry concepts. The application of the RGB-12 algorithm provided very good red/green (AGREEprep)/blue/white scores (%) of 100/80/98/93, determined primarily by in-situ DGT passive sampling, very good detection limits and cost-effective SSETV-µCCP-OES instrumentation.

Preparation, Characterization, and Performance of Natural Zeolites as Alternative Materials for Beer Filtration.

The clarity of the beer is essential to its marketability and good consumer approval. Moreover, the beer filtration aims to remove the unwanted constituents that cause beer haze formation. Natural zeolite, an inexpensive and widespread material, was tested as a substitute filter media for diatomaceous earth in removing the haze constituents in beer. The zeolitic tuff samples were collected from two quarries in Northern Romania: Chilioara, in which the zeolitic tuff has a clinoptilolite content of about 65%, and the Valea Pomilor quarry, containing zeolitic tuff with a clinoptilolite content of about 40%. Two-grain sizes, <40 and <100 µm, from each quarry were prepared and thermally treated at 450 °C in order to improve their adsorption properties and remove organic compounds and for physico-chemical characterization. The prepared zeolites were used for beer filtration in different mixtures with commercial filter aids (DIF BO and CBL3) in laboratory-scale experiments, and the filtered beer was characterized in terms of pH, turbidity, color, taste, flavor, and concentrations of the major and trace elements. The results showed that the taste, flavor, and pH of the filtered beer were generally not affected by filtration, while turbidity and color decreased with an increase in the zeolite content used in the filtration. The concentrations of Na and Mg in the beer were not significantly altered by filtration; Ca and K slowly increased, while Cd and Co were below the limits of quantification. Our results show that natural zeolites are promising aids for beer filtration and can be readily substituted for diatomaceous earth without significant changes in brewery industry process equipment and protocols for preparation.

Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581.

This study aimed to analyze the production of poly(3-hydroxybutyrate) (PHB) from lignocellulosic biomass through a series of steps, including microwave irradiation, ammonia delignification, enzymatic hydrolysis, and fermentation, using the Bacillus megaterium ATCC 14581 strain. The lignocellulosic biomass was first pretreated using microwave irradiation at different temperatures (180, 200, and 220 °C) for 10, 20, and 30 min. The optimal pretreatment conditions were determined using the central composite design (CCD) and the response surface methodology (RSM). In the second step, the pretreated biomass was subjected to ammonia delignification, followed by enzymatic hydrolysis. The yield obtained for the pretreated and enzymatically hydrolyzed biomass was lower (70.2%) compared to the pretreated, delignified, and enzymatically hydrolyzed biomass (91.4%). These hydrolysates were used as carbon substrates for the synthesis of PHB using Bacillus megaterium ATCC 14581 in batch cultures. Various analytical methods were employed, namely nuclear magnetic resonance (1H-NMR and13C-NMR), electrospray ionization mass spectrometry (EI-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA), to identify and characterize the extracted PHB. The XRD analysis confirmed the partially crystalline nature of PHB.

Optimization of Lipid Extraction from Spirulina spp. by Ultrasound Application and Mechanical Stirring Using the Taguchi Method of Experimental Design.

The present study uses the Taguchi method of experimental design to optimize lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring. A Taguchi L9 orthogonal array was used to optimize various parameters, such as methanol: chloroform (M:C) ratio, biomass: solvent ratio, and extraction time for lipid extraction. The results were analyzed using the signal-to-noise (S/N) ratio and analysis of variance (ANOVA). The biomass: solvent ratio significantly influenced lipid content (p < 0.05) with 92.1% and 92.3% contributions to the lipid and S/N ratio data, respectively. The extraction time presented a contribution value of 5.0%, while the M:C ratio presented the most negligible contribution of 0.4% for S/N data. The optimum extraction conditions were: M:C ratio of 1:1, biomass: solvent ratio of 1:60, and extraction time of 30 min. The predominant fatty acids were palmitic acid (44.5%), linoleic acid (14.9%), and gamma-linolenic acid (13.4%). The confirmation experiments indicated a lipid content of 8.7%, within a 95% confidence interval, proving the Taguchi method's effectiveness in optimizing the process parameters for lipid extraction.

Zeolites Reduce the Transfer of Potentially Toxic Elements from Soil to Leafy Vegetables.

The ability of natural zeolite amendment to reduce the uptake of potentially toxic elements (PTEs) by lettuce, spinach and parsley was evaluated using pot experiments. PTE concentrations in roots and shoots, as well as the pseudo total (PT), water soluble (WS) and bioavailable (BA) PTE fractions in the amended soils, were assessed. Although the PT PTE concentration was high, the WS fraction was very low (<0.4%), while the BA fraction varied widely (<5% for Cr, Mn and Co, <15% for Ni, Pb and Zn, >20% for Cd and Cu). PTE concentration decreased in both roots and shoots of all leafy vegetables grown on zeolite amended soils, especially at high amendment dose (10%). The uptake of PTEs mainly depended on plant species, PTE type and amendment dose. With the exception of Zn in spinach, the bioaccumulation factor for roots was higher than for shoots. Generally, lettuce displayed the highest PTE bioaccumulation capacity, followed by spinach and parsley. Except for Zn in spinach, the transfer factors were below 1 for all PTEs, all plant species and all amendment doses. Our results showed that the natural zeolites are promising candidates in the reclamation of contaminated soils due to their ability to immobilize PTEs.

Synthesis and Phase Behavior of a Platform of CO2-Soluble Functional Gradient Copolymers Bearing Metal-Complexing Units.

The synthesis and characterization of a platform of novel functional fluorinated gradient copolymers soluble in liquid and supercritical CO2 is reported. These functional copolymers are bearing different types of complexing units (pyridine, triphenylphosphine, acetylacetate, thioacetate, and thiol) which are well-known ligands for various metals. They have been prepared by reversible addition-fragmentation chain-transfer (RAFT) polymerization in order to obtain well-defined gradient copolymers. The copolymers have been characterized by proton nuclear magnetic resonance (1H-NMR) spectroscopy, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, thermal gravimetric analysis (TGA), dynamical scanning calorimetry (DSC) and cloud point measurements in dense CO2. All the investigated metal-complexing copolymers are soluble in dense CO2 under mild conditions (pressure lower than 30 MPa up to 65 °C), confirming their potential applications in processes such as metal-catalyzed reactions in dense CO2, metal impregnation, (e.g., preparation of supported catalysts) or metal extraction from various substrates (solid or liquid effluents). Particularly, it opens the door to greener and less energy-demanding processes for the recovery of metals from spent catalysts compared to more conventional pyro- and hydro-metallurgical methods.

Simultaneous Removal of Heavy Metals (Cu, Cd, Cr, Ni, Zn and Pb) from Aqueous Solutions Using Thermally Treated Romanian Zeolitic Volcanic Tuff.

Increased concentrations of heavy metals in the environment are of public health concern, their removal from waters receiving considerable interest. The aim of this paper was to study the simultaneous adsorption of heavy metals (Cu, Cd, Cr, Ni, Zn and Pb) from aqueous solutions using the zeolitic volcanic tuffs as adsorbents. The effect of thermal treatment temperature, particle size and initial metal concentrations on the metal ions sorption was investigated. The selectivity of used zeolite for the adsorption of studied heavy metals followed the order: Pb > Cr > Cu > Zn > Cd > Ni. The removal efficiency of the heavy metals was strongly influenced by the particle sizes, the samples with smaller particle size (0−0.05 mm) being more efficient in heavy metals removal than those with larger particle size (1−3 mm). Generally, no relevant changes were observed in heavy metals removal efficiency for the treatment temperatures of 200 °C and 350 °C. Moreover, at a higher temperature (550 °C), a decrease in the removal efficiencies was observed. The Cd, Zn, Cu, Cr, Zn and Ni sorption was best described by Langmuir model according to the high values of correlation coefficient. The pseudo-first-order kinetic model presented the best correlation of the experimental data.

Characteristics of Volcanic Tuff from Macicasu (Romania) and Its Capacity to Remove Ammonia from Contaminated Air.

In the present work, the capability of the volcanic tuff from Macicasu (Romania) to remove ammonia (NH3) from air with different contamination levels during 24 h of adsorption experiments was investigated. The natural zeolitic volcanic tuff was characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), the Brunauer-Emmett-Teller (BET) method, inductively coupled plasma optical emission spectrometry (ICP-OES), and thermogravimetric analysis (TGA). The adsorption capacities varied between 0.022 mg NH3 g-1 zeolite and 0.282 mg NH3 g-1 zeolite, depending on the NH3 concentrations in the air and at the contact time. The nonlinear forms of the Langmuir and Freundlich isotherm models were used to fit the experimental data. Additionally, the adsorption of NH3 was studied using nonlinear pseudo-first-order (PFO), pseudo-second-order (PSO), and Elovich kinetic model. Based on the total volume of pores of used volcanic tuff, the NH3 was removed from the air both due to the physical adsorption of NH3 gas and the ion exchange of NH4+ (resulted from a reaction between NH3 and H2O adsorbed by the zeolite). Depending on the initial NH3 concentration and the amount of volcanic tuff, the NH3 concentrations can be reduced below the threshold of this contaminant in the air. The adsorption capacity of NH3 per unit of zeolite (1 g) varied in the range of 0.022-0.282 mg NH3 g-1 depending on the NH3 concentration in the air.

The Potential Application of Natural Clinoptilolite-Rich Zeolite as Support for Bacterial Community Formation for Wastewater Treatment.

The aim of this study was to investigate the use of natural zeolite as support for microbial community formation during wastewater treatment. Scanning electron microscopy (SEM), thermal decomposition and differential thermogravimetric curves (TGA/DGT) techniques were used for the physicochemical and structural characterization of zeolites. The chemical characterization of wastewater was performed before and after treatment, after 30 days of using stationary zeolite as support. The chemical composition of wastewater was evaluated in terms of the products of nitrification/denitrification processes. The greatest ammonium (NH4+) adsorption was obtained for wastewater contaminated with different concentrations of ammonium, nitrate and nitrite. The wastewater quality index (WWQI) was determined to assess the effluent quality and the efficiency of the treatment plant used, showing a maximum of 71% quality improvement, thus suggesting that the treated wastewater could be discharged into aquatic environments. After 30 days, NH4+ demonstrated a high removal efficiency (higher than 98%), while NO3+ and NO2+ had a removal efficiency of 70% and 54%, respectively. The removal efficiency for metals was observed as follows (%): Mn > Cd > Cr > Zn > Fe > Ni > Co > Cu > Ba > Pb > Sr. Analysis of the microbial diversity in the zeolite samples indicated that the bacteria are formed due to the existence of nutrients in wastewater which favor their formation. In addition, the zeolite was characterized by SEM and the results indicated that the zeolite acts as an adsorbent for the pollutants and, moreover, as a support material for microbial community formation under optimal conditions. Comparing the two studied zeolites, NZ1 (particle size 1−3 mm) was found to be more suitable for wastewater treatment. Overall, the natural zeolite demonstrated high potential for pollutant removal and biomass support for bacteria community growth in wastewater treatment.

Exploring the Properties of Micronized Natural Zeolitic Volcanic Tuff as Cosmetic Ingredient.

This study explores the characteristics of a micronized natural zeolitic volcanic tuff (MZ) as ingredient in cosmetic formulations. In particular, the purpose was to prepare and investigate the organoleptic and physicochemical properties of two representative cosmetic formulations containing MZ. The MZ samples were characterized using X-ray fluorescence (XRF), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. pH, cation exchange capacity (CEC), apparent density, chemical composition and particle size distribution of MZ samples were also determined. The micronization treatment applied did not produce significant structural and physicochemical changes with respect to the raw zeolitic volcanic tuff. The prepared formulations containing 5% MZ with different particle sizes (100-125 µm and 125-250 µm) were subjected to preliminary and accelerated stability tests, and the pH and organoleptic properties were also evaluated. The cosmetic formulations presented a pH of 4.3, a pleasant touch, good spreadability, easy application on skin, no color alteration and a good stability after 15, 30 and 60 days of storage at room temperature, low temperature and freezer during the accelerated stability tests. The obtained results endorse the MZ as suitable for the development of formulations exploiting the clinoptilolite properties as a cosmetic ingredient.

Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues.

Gold nanoparticles (AuNPs) have a wide-ranging application and are widespread in samples with complex matrices; thus, efficient analytical procedures are necessary to identify and characterize this analyte. A sensitive analytical method for determination of AuNPs content in biological tissues, based on microwave-assisted acid wet digestion and graphite furnace atomic absorption spectrometry (GFAAS) validated in accordance with the requirements of Eurachem guideline and ISO 17025 standard, is presented in this study. The digestion procedure was optimized, and the figures of merit such as selectivity, limit of detection (0.43 µg L-1), limit of quantification (1.29 µg L-1, corresponding to 12.9 µg kg-1 in tissue sample, considering the digestion), working range, linearity, repeatability ((RSDr 4.15%), intermediate precision (RSDR 8.07%), recovery in accuracy study (97%), were methodically evaluated. The measurement uncertainty was assessed considering the main sources of uncertainties and the calculated relative expanded uncertainty (k = 2) was 12.5%. The method was applied for the determination of AuNPs in six biological tissues (liver, small intestine, heart, lungs, brain and kidneys) and the found concentrations were generally at low levels, close or lower than LOQ.

Application of Inductively Coupled Plasma Spectrometric Techniques and Multivariate Statistical Analysis in the Hydrogeochemical Profiling of Caves-Case Study Cloșani, Romania.

The aim of the study was to develop the hydrogeochemical profiling of caves based on the elemental composition of water and silty soil samples and a multivariate statistical analysis. Major and trace elements, including rare earths, were determined in the water and soil samples. The general characteristics of water, anions content, inorganic and organic carbon fractions and nitrogen species (NO3- and NH4+) were also considered. The ANOVA-principal component analysis (PCA) and two-way joining analysis were applied on samples collected from Cloșani Cave, Romania. The ANOVA-PCA revealed that the hydrogeochemical characteristics of Ca2+-HCO3- water facies were described by five factors, the strongest being associated with water-carbonate rock interactions and the occurrence of Ca, Mg and HCO3- (43.4%). Although organic carbon fractions have a lower influence (20.1%) than inorganic ones on water characteristics, they are involved in the chemical processes of nitrogen and of the elements involved in redox processes (Fe, Mn, Cr and Sn). The seasonal variability of water characteristics, especially during the spring, was observed. The variability of silty soil samples was described by four principal components, the strongest influence being attributed to rare earth elements (52.2%). The ANOVA-PCA provided deeper information compared to Gibbs and Piper diagrams and the correlation analysis.

Immobilization of Potentially Toxic Elements in Contaminated Soils Using Thermally Treated Natural Zeolite.

Rehabilitation of contaminated soils is a complex and time-consuming procedure. One of the most cost-effective and easy-to-use soil remediation approaches is the use of amendments that stabilize the potential toxic elements (PTE) in soil by reducing their mobility and bioavailability. The stabilization of Cu, Pb, Zn, Cd, Co, Cr, Ni in a contaminated soil using 5% and 10% amendment with thermally treated natural zeolite was investigated using a sequential extraction procedure, contamination and environmental risk factors. The results showed that after amendment, the PTE concentration decreased in the exchangeable and reducible fractions and increased in the oxidizable and residual fractions. The highest immobilization effect, consisting in the decrease of exchangeable fractions with 69% was obtained in case of 10% zeolite amendment and 90 days of equilibration time for Pb; also, more than half of the mobile fraction was immobilized in case of Zn, Cu, and Co and about one third in case of Ni, Cr, and Cd. Generally, the immobilization effect of the 5% and 10% amendment is comparable, but a higher equilibration time enhanced the immobilization effect, especially in the case of Cd, Co, Cu, Pb, and Zn.